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Human Genetics

HOD Profile

Dr. Solomon FD Paul
Dr. Solomon FD Paul
M.Sc., Ph.D
Human Genetics
Research Areas
Radiobiology, Radiation genetics, Biodosimetry, Biophysics and Environmental Biology
Awards and Recognitions
1)Inter Institute Research Collaboration – University of Miami, Florida, USA, May 2010 2)Sri Ramachandra University, Certificate of Merit, September 2008 3)Sri Ramachandra University Institutional Research Award for establishing the Biodosimetry Laboratory, September 2007 4)Inter Institute Research Collaboration Berlin Germany, December 2004 5)Dr. A.R. Gopal Ayengar Young Scientist Award 2001 given to outstanding work carried out in the field of Radiation Biology, Biophysics and Environmental Biology, February 2001
Membership in Committees
1.Board of Studies Member 2.Academic Senate and Research Advisory Committee member 3.National Assessment and Accreditation Council (NAAC) - Steering Committee member Professional Memberships 1.ISRP - (Indian Society of Radiation Protection) IGCAR, Kalpakkam – India. 2.IARP - (Indian Association for Radiation Protection) IGCAR, Kalpakkam - India 3.EMSI - (Environmental Mutagen Society of India) BARC, Mumbai-India 4.ISHG - (India Society of Human Genetics) 5.ISRB – (Indian Society of Radiation Biology) BARC, Mumbai-India 6.CITI – (ETHICS)
Biological Dosimetry
The signatures left behind by ionising radiation on the DNA and chromosome as it traverses through the cells are very good stable indicators of radiation absorbed dose. Various batteries of assays like the prodromal, biochemical, physiological, cytogentetical and molecular biodosimeters have been used to estimate radiation dose. Each biodosimeter does carry some good qualities like the sensitivity, stability, reliability, ability to differentiate partial from whole body, internal from external exposure and so forth. These assays can quantify the radiation dose received by a person above a minimum threshold with 95% precision and the methodology has been perfected with good confidence and standards. The damage that is incurred by any living cell can be observed by diverse techniques tailored for specific end points referred to as biomarkers. With a host of assays available, it is difficult to say which assay is the best. No single biodosimetric technique meets all the requirements of an ideal biodosimeter and thus qualifies as a "gold standard." An approach of using combined dosimetry from the most appropriate methods in a given situation has been advocated. However cytogenetic methods of dose determination, have dominated the biodosimetry arena due to their precise and increasingly informative nature, in the time immediately following exposure. It should be noted, however, that some of these more complex analyses may require a high degree of expertise. The pre-requisite for any laboratory involved in the radiation dose assessment is to establish a reference dose response curve with known doses of radiation exposure. Then, the absorbed dose of unknown can be extrapolated from the yield of aberration frequency obtained from any exposed individual using the reference curve. It becomes important for every laboratory working on biological dosimeters to develop their own in vitro and in vivo dose response curves. The department of Human Genetics at Sri Ramachandra University has developed various in vitro dose response curves for various types of ionizing radiation using chromosomal aberration (CA), micronuclei (MN), comet assay, FISH (partial whole chromosome probes, mFISH probes, centromeric, & telomeric probes). We have investigated the use of the FISH, glycophorin-A (GPA), T-cell receptor (TCR) and comet assays for estimating equivalent whole body doses in cancer patients receiving radiotherapy. The era of high through put equipment like metaphase finder, rare cell detection, flow cytometry etc. will enable us to further improve on the qualities like the speed and large sample number. A much sensitive level of dose estimate with much more precise dose estimate can be derived from the 95% confidence dose response curve by scoring more number of cells even at very low doses.
Studies on the Radio-protective effect of 2-DG an inhibitor of anaerobic glycolysis, to study radio-protective effect in human peripheral blood lymphocytes from normal persons and cervical cancer patients exposed in-vivo to high dose-rate gamma radiation as indicated by chromosomal aberrations and micronuclei assay. The obtained results showed that 2-DG showed a protection in normal lymphocytes and sensitize the same in blood samples obtained from cervical cancer patients.
To determine whether the bystander effects induced by chemotherapeutic agents are similar to those induced by ionising radiation and to analyse the cell dependency, if any, in different human cell types such as normal lung fibroblasts (WI-38), human bone marrow mesenchymal stem cells (hBMSC), lung adenocarcinoma (A-549, NCI-H23) and peripheral blood lymphocytes (PBL).
Cancer Genetics
Genetic alterations serve as an important risk factor for the occurrence and progression of the cancer. The heterogeneous nature of prostate cancer reflects the multiple genetic and environmental factors important for its etiology. Androgens play a very important role in the growth and maintenance of the prostate gland. Genetic polymorphisms that encode for key enzymes involved in androgen biosynthesis and metabolism have been of much epidemiological interest in their relation to hormone-dependent cancer risk. Work at the department of Human Genetics focused on the polymorphism of genes involved in androgen metabolism in South Indian population has been focused on prostate cancer, the sixth most common cancer in the world and the third most common cancer in men, accounts for 9•7% of all cancers in men (15•3% in developed countries and 4•3% in developing countries). To understand the genetics of prostate carcinogenesis and the racial variation in prostate cancer incidence it is essential to determine the relationship of the polymorphic markers of each gene individually within each racial ethnic group. This will help us to understand the relationship between each gene and prostate cancer, the polygenic etiology of prostate cancer and the extent that the variation in these genes individually and collectively explains racial-ethnic variation in prostate cancer occurrence
Cardiomyocyte and Chondrocytes Cultures
Culturing of cardiomyocyte and chondrocytes cells in-vitro on specific scaffolds. These would find potential use in regenerative medicine and several works including characterization has been studied.